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. 2021 Jun 15;12:661939. doi: 10.3389/fimmu.2021.661939

Figure 1.

Figure 1

NLRP3 inflammasome is over-expressed and highly activated in AML, which plays leukemia-promoting effects in vitro. (A) The western blot results of NLRP3, NF-κB, caspase-1, pro-IL-1β and ASC in BM-MNCs from ND AML patients (n=3) and controls (n=3). (B) The concentrations of IL-1β and IL-18 in BM supernatant from ND AML patients (n=70) in comparison to controls (n=15). (C) The Western blot results of pNF-κB, pro-caspase-1, pro-IL-1β, cleaved caspase-1 and cleaved IL-1β were showed in primary leukemia cells after different treatment. β-actin is used as a loading control. (D) LPS stimulated the secretion of IL-1β and IL-18 into the supernatants from cultured leukemia cells. (E) CCK8 analysis was performed to detect the proliferation of leukemia cells 24, 48 and 72 hours after LPS stimulation (n=10). (F) The quantified apoptosis rate was shown (n=10). (G) The western blot results of PARP, C-myc and Bcl-2 in primary leukemia cells after LPS stimulation. (H) CCK8 analysis for the proliferation of primary leukemia cells with or without LPS 24, 48 and 72 hours after treatment with ADR, DNR (n=10). (I) The apoptosis results of primary leukemia cells with or without LPS after treatment with ADR, DNR (n=6). (J) The expression of NLRP3 in U937 cell line was enhanced after being transfected with lentivirus (n=3). (K) The IC50 value of ADR for U937 cells was higher after upregulating NLRP3 expression (n=3). (L) PARP, C-myc and Bcl-2 in primary leukemia cells after LPS stimulation (n=1). β-actin is used as a loading control. *P < 0.05; **P < 0.01; ***P < 0.001.