Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 15;12:639189. doi: 10.3389/fpls.2021.639189

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Zhu, Shi and Jie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Multiple sequence alignment and phylogenetic analysis of BnPCS1 and PCS proteins from other plant species. (A) Alignment of BnPCS1 and its orthologous proteins from various plant species constructed using DNAMAN 8.0 software. The shaded regions indicate the conserved amino acid residues (those shown in black are fully conserved; the amino acids highlighted in pink and light green are similar). The region underlined in black is the phytochetatin domain, and the phytochelatin_C domain is underlined in red. The three active sites are indicated by black arrows. The C³⁷¹C³⁷²QETC³⁷⁶VKC³⁷⁹ motif is enclosed in a black box. (B) Phylogenetic tree showing the evolutionary relationships between BnPCS1 and 18 other PCS proteins from various plant species constructed using the neighbor-joining (NJ) method in MEGA 5.0. The scale bar represents 0.05 amino acid substitutions per site. GenBank accession numbers of the proteins are as follows: AtPCS1 (AAD16046.1) and AtPCS2 (AAK94671.1) from Arabidopsis thaliana, BjPCS1 (CAC37692.1) from Brassica juncea, TjPCS1 (BAB93119.1) from Thlaspi japonicum, NcPCS1 (BAB93120.1) from Noccaea caerulescens, PbPCS1 (AEY68568.1) from Pyrus betulifolia, LjPCS1 (AAT80342.1) from Lotus japonicus, GmPCS1 (AAL78384.1) from Glycine max, LsPCS1 (AAU93349.1) from Lactuca sativa, SaPCS1 (ACU44656.1) from Sonchus arvensis, NtPCS1 (AAO74500.1) from Nicotiana tabacum, StPCS1 (CAD68110.1) from Solanum tuberosum, AsPCS1 (AAO13809.1) from Allium sativum, CdPCS1 (AAO13810.2) from Cynodon dactylon, TaPCS1 (AAD50592.1) from Triticum aestivum, OsPCS1 (AAO13349.2) from Oryza sativa, and PvPCS1 (AAT11885.1) and AyPCS1 (BAB64932.1) from the ferns Pteris vittata and Athyrium yokoscense, respectively.