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. 2021 Jun 15;12:689104. doi: 10.3389/fpls.2021.689104

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Copyright © 2021 Shin, König-Beihammer, Vavra, Schwestka, Kienzl, Klausberger, Laurent, Grünwald-Gruber, Vierlinger, Hofner, Margolin, Weinhäusel, Stöger, Mach and Strasser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Human CRT retains glycoproteins in intracellular compartments. (A) HsCRT was co-expressed with RBD and analyzed 4 days after infiltration of Nicotiana benthamiana WT by immunoblotting with antibodies against RBD or the 6x histidine tag. (B) Endo H digestion of RBD co-expressed with. HsCRT.(C) Confocal microscopy of RBD-RFP co-expressed with HsCRT, AtCNX1 or AtCRT2. Scale bars = 50 μm. (D) Enlarged confocal image of RBD-RFP co-expressed with HsCRT. (E) Endo H digestion and immunoblotting of RBD-RFP co-expressed with HsCRT. (F) RFP-PDI5 co-expressed with/without HsCRT, HEXO3-RFP co-expressed with/without HsCRT, ST-RFP co-expressed with/without HsCRT. Images were taken 3 days after infiltration. Scale bars = 10 μm.