Fig. 4.

a Relative mRNA expression level of MAP4K1 in nine AML cell lines (expression of MAP4K1 in other cell lines compared with THP-1). b Westernblot analysis of the protein level of MAP4K1 in nine AML cell lines. c Cell viability after MAP4K1 knockdown in AML cell line THP-1 and MV4-11 R50 were measured by MTS assay [two-tailed Student's t-tests; *P < 0.05, **P < 0.01, ***P < 0.001]. d Cell viability after MAP4K1 overexpression in AML THP-1 and MV4-11 cell lines as determined by MTS assay [two-tailed Student's t-tests; *P < 0.05, **P < 0.01]. e The percentage of cells in the G1, S, or G2 phase in MAP4K1 knockdown cell lines for 48 h was detected by flow cytometry [two-tailed Student's t-tests; *P < 0.05, **P < 0.01]. f Percentage of cells in the G1, S, or G2 phase in MAP4K1 overexpressed cell lines for 48 h was detected by flow cytometry. g Cell lysates after MAP4K1 knockdown were collected, and levels of cell cycle-related proteins P21 and P27 were determined by Western blotting with the respective antibodies. h Cell lysates after MAP4K1 overexpression were collected, and the cell cycle-related proteins P21, P27 were examined by Western blotting with the respective antibodies. Western Blot bands were derived from three separate experiments but only one representative loading control is shown. Each value indicates the mean ± SEM of three or more independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.