Fig. 5.

a Molecules in MAPK pathway (refer to ProteinLounge.com). b Protein levels of MAP4K1, JNK, C-JUN in AML cell lines. Total and phosphorylated JNK and C-JUN expression were downregulated after MAP4K1 knockdown while these two proteins were upregulated after MAP4K1 overexpression. c DNA repair and damage pathways were significantly dysregulated after MAP4K1 overexpression via bioinformatics analysis (UP panel: GO enrichment analysis, DOWN panel: GSEA analysis). d Several main pathways which had changed as shown by GSEA analysis. e Maker protein P-H2AX in DNA damage pathway up-regulated after MAP4K1 knockdown (e, left) and down-regulated after MAP4K1 overexpression (e, right) while DNA repair proteins have the opposite trend. f Possible mechanism of MAP4K1 in AML: MAP4K1 plays a biological role in AML through MAPK&DNA damage/repair pathways. These pathways modulated the progression of the cell cycle in AML through P21/P27 and ultimately influenced the progress of AML. Western Blot bands were derived from three separate experiments but only one representative loading control.