. 2021 May 27;297(1):100836. doi: 10.1016/j.jbc.2021.100836

Table 1.

Maximal binding capacity (B_max) and dissociation constants (K_d) for binding of prey proteins, hS2, to bait proteins, hC0-C2, with or without PKA in the SPBA experiments

Prey	Bait	n	B_max (mol/mol: S2/C0-C2)	K_d (μM)
hS2^Wt	hC0-C2	3	1.22 ± 0.02	5.04 ± 0.33
hS2^R870H		3	0.93 ± 0.04∗∗	6.29 ± 0.53
hS2^E924K		3	0.96 ± 0.02∗	4.30 ± 0.17
hS2^E930Δ		3	0.94 ± 0.05∗∗	6.67 ± 0.48
hS2^Wt	hC0-C2p	3	0.92±0.03∗∗	7.35 ± 0.56∗∗
hS2^R870H		3	0.76±0.05∗∗∗	1.26 ± 0.17∗∗∗^{, §§§, ¶¶¶}
hS2^E924K		3	0.73±0.07∗∗∗	0.83 ± 0.13∗∗∗^{, §§§, ¶¶¶}
hS2^E930Δ		3	0.70±0.04∗∗∗	0.97 ± 0.08∗∗∗^{, §, ¶¶¶}

Data are expressed as the mean ± SEM for the observed values. Statistical analyses were performed in all groups using ordinary one-way ANOVA, followed by Tukey’s multiple comparison test with single pooled variance. See Table 7 for analysis of main factors and interactions. ‘n’ is the times binding experiments were performed independently in replicates of three.

∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus hS2Wt /hC0-C2.

^§p < 0.05, ^§§p < 0.01, ^§§§p < 0.001 versus hS2Wt/hC0-C2p.

^¶p < 0.05, ^¶¶p < 0.01, ^¶¶¶p < 0.001 versus respective mutant/hC0-C2.