Table 2.

Maximal binding capacity (B_max) and dissociation constants (K_d) for binding of prey proteins, hS2, to bait proteins, hC0-C2, with phospho-ablation and phospho-mimetic in the SPBA experiments

Prey	Bait	n	Bmax (mol/mol S2/C0-C2)	Kd (μM)
hS2Wt	mC0-C2	3	1.02 ± 0.04	1.89 ± 0.03
hS2R870H		3	0.75 ± 0.01∗∗	1.64 ± 0.34
hS2E924K		3	0.71 ± 0.05∗∗	1.51 ± 0.29
hS2E930Δ		3	0.70 ± 0.01∗∗	1.32 ± 0.21
hS2Wt	mC0-C2AAA	3	0.95 ± 0.04	1.81 ± 0.32
hS2R870H		3	0.74 ± 0.03∗∗,§	1.51± 0.22
hS2E924K		3	0.67 ± 0.04∗∗,§	1.48 ± 0.24
hS2E930Δ		3	0.70 ± 0.02∗∗,§	1.22 ± 0.13
hS2Wt	mC0-C2DDD	3	0.70 ± 0.04∗∗,§§	2.02 ± 0.56
hS2R870H		3	0.70 ± 0.04∗∗,§	0.79 ± 0.19¶
hS2E924K		3	0.73 ± 0.11∗∗,§	0.74 ± 0.21∗,¶
hS2E930Δ		3	0.68 ± 0.02∗∗,§	0.88 ± 0.10∗∗,§,¶¶

Statistical significance was calculated by performing ordinary one-way ANOVA with Tukey’s multiple comparison test with single pooled variance. ‘n’ is the times binding experiments were performed independently in replicates of three. ± values represent the SEM for the measurements. See Table 7 for analysis of main factors and interactions.

∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus hS2^Wt/mC0-C2.

^§p < 0.05, ^§§p < 0.01, ^§§§p < 0.001 versus hS2^Wt/mC0-C2^AAA.

^¶p < 0.05, ^¶¶p < 0.01, ^¶¶¶p < 0.001 versus hS2^Wt/mC0-C2^DDD.