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. 2021 May 7;7(5):459–470. doi: 10.1002/cjp2.220

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© 2021 The Authors. The Journal of Pathology: Clinical Research published by The Pathological Society of Great Britain and Ireland & John Wiley & Sons, Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Assessment of IHC and ISH for the identification of SARS‐CoV‐2‐infected cells. (A–C) Positive control cell block made of SARS‐CoV‐2‐infected VERO cells, with hematoxylin and eosin (A, ×200 magnification), IHC using the Thermo‐Fisher Scientific monoclonal antibody (B, ×200 magnification), and RNAScope ISH (C, ×200 magnification). (D) Paired region analysis showing the distribution of virus‐infected cells is comparable between IHC (upper panel) and ISH (lower panel) (×100 magnification). (E) Paired region analysis showing that histopathologic changes associated with COVID‐19 (upper panel) may be negative for virus‐infected cells by IHC (lower panels) (×20 magnification). (F) Paired region analysis showing relatively bland areas (upper panel) may have many virus‐infected cells by IHC (lower panel) (F, ×20 magnification; inset ×200 magnification). All magnifications represent total magnification.