Fig 5.

Effect of IL‐6 on fibrinolysis. (A) Clot formation and lysis were assayed by thrombelastography (TEG). ²⁵ Human blood pooled from six healthy volunteers collected in citrate was pre‐incubated with or without IL‐6 (50 pg/ml) for one hour. Tissue plasminogen activator (tPA; 10 nM) was added to samples preincubated with IL‐6 (tPA+IL‐6) or without IL‐6 (tPA) immediately before kaolin was added to initiate coagulation. ²⁵ The results shown are representative of three independent experiments. (B) Fibrinolysis (Ly) was calculated from experiments performed as in (A) and in triplicate in the presence of tPA alone (tPA) or tPA and IL‐6 (tPA+IL‐6). In other experiments, α‐defensin (64 ng/ml) was added instead of IL‐6 (tPA+ α‐Def). In a third set of experiments, tPA was added together with IL‐6 and colchicine (10 nM; tPA+IL‐6+Colch) or with α‐defensin and colchicine (10 nM; tPA+ α‐Def+Colch) or colchicine alone (Colch). The results (mean ± SD) were calculated from three independent experiments, each performed in triplicate. MA, maximal amplitude. [Colour figure can be viewed at wileyonlinelibrary.com]