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. 2021 Jun 20;15:100358. doi: 10.1016/j.ynstr.2021.100358

Fig. 2.

Fig. 2

The activity of 6–8-week-old new mature aDGCs regulates spatial memory, anxiety-like behavior, as well as the excitability of mature and immature aDGCs. a, Experimental design for the behavioral tests. BMT, Barnes maze test; OFT, open field test; EPMT, elevated plus maze test; and TST, tail suspension test. b, The heatmap of mice in probe test 2 of BMT. c, The latency to the escape hole in BMT during training and in probe tests 1 and 2 (two-way ANOVA, interaction F12,265 = 0.723, P = 0.729; day F6,265 = 5.633, *P < 0.001; virus F2,265 = 0.095, P = 0.909). d, Active time at the escape hole (F2,38 = 4.653, *P = 0.016). e, The heatmap of mice in OFT. f, Active time of mice in the center of OFT (F2,36 = 4.422, *P = 0.019). g, Total distance traveled in OFT (F2,36 = 0.749, P = 0.480). h, Heatmap of mice in EPMT. i, Percentage of active time in the open arms of EPMT (F2,37 = 2.409, P = 0.104, planned comparison t-test, hM3Dq versus mCherry, *P = 0.013). j, Percentage of entries into the open arms of EPMT (F2,37 = 2.481, P = 0.097, planned comparison t-test, hM3Dq versus mCherry, *P = 0.032). k and l, Total time spent immobile in TST (F2,37 = 0.891, P = 0.419). m-q, Representative images, and quantification of c-Fos and Egr1 expression, and DCX-positive immature aDGCs in the DG (o, F2,10 = 8.969, *P = 0.006; p, F2,18 = 4.628, *P = 0.024; q, F2,15 = 2.885, P = 0.087). All scale bars are 100 μm.