Figure 6. The impact of cisplatin on cell cycle regulation in PC9 cells. (A) PC9 cells were treated with DMSO or cisplatin (20 nM, 100 nM and 200 nM) for 72 h and stained with propidium iodide (PI) before a flow cytometric analysis. Representative results from three independent experiments are shown. (B) The results are presented as the mean±SD of three independent experiments. ***p<0.001 compared with the DMSO-treated group, using one-way ANOVA with Bonferroni’s multiple comparison test. (C) For the Western blot analysis, PC9 cells were treated with DMSO or cisplatin (20 nM, 100 nM, 200 nM) for 72 h. β-actin served as a loading control. Representative immunoblots from three independent trials are shown.