Figure 5.

(A) Multiplex detection of anti-Dig (blue) and cetuximab (orange) antibodies using two orthogonal-responsive circuits releasing two different reporter strands labeled with two non-interfering redox labels (i.e., methylene blue, MB, and anthraquinone, AQ). (B) SWV profiles achieved for different experiments performed in the absence of both antibodies (left), in the presence of only one antibody (center), and in the presence of both antibodies (right). The colored antibody image identifies the added target for each experiment. The experiments were performed in a 100 μL phosphate buffer (50 mM Na₂HPO₄, 150 mM NaCl, pH 7.0) solution containing the two antibody-responsive circuits as previously described. The SWV voltammograms were obtained at saturating concentrations of the target antibody (300 nM).