Table 2.

stock conc.	5 M NaCl	1 M KCl	1 M MgSO4·7H2O	0.5 M MES	6.7 mM nigericin	monensin (1000×)	1 N KOH/1 N HCl	final volume
volume	10 μL	1150 μL	13 μL	500 μL	15 μL	10 μL	a	10 mL
final conc.	5 mM	115 mM	1.3 mM	25 mM	10 μM	1×		(H2O)

^apH values are adjusted using a pH meter with appropriate acid/base (conc, concentration).