Figure 1.
Conceptual sketch of the microfluidic system for antibiotic susceptibility testing. The platform consists of four sets of chamber arrays with hundreds of growth chambers (300 × 300 × 75 μm3) fabricated using the air-tight material, cyclic olefin copolymer. The chambers are filled with oxygen-sensing nanoprobes and different concentrations of the studied antibiotics embedded in the agarose gel. Changes in the metabolic activity of the bacteria result in changes in the luminescence signal of the nanoprobes, enabling the determination of susceptibility or resistance of the studied pathogens against the various antibiotic compounds.