Fig. 4.

Alpha and beta diversity metrics of seed endophyte microbiota. (A) Shannon–Weaver, Simpson, Dominance and Equitability indices of bacterial microbiota (OTU 97%), grouped by cultivation form (t-test, P < 0.05), according to 16S rRNA gene metabarcoding. CS = Cultivated species; WS = Wild species. (B) Non-metric multidimensional scaling plot for bacterial microbiota structure based on weighted Bray-Curtis distances. Samples are colored by plant species and shaped by cultivation form. ADONIS significance test: R² = 0.078, P = 0.003 for the factor “cultivation form”; R² = 0.32, P < 0.001 for the factor “species”; stress value: 0.1495.