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. 2021 Jan 20;31:137–153. doi: 10.1016/j.jare.2021.01.007

Fig. 8.

Fig. 8

Representative histogram showing the flow cytometric analysis of Annexin V-FITC staining in non-infected control (a) and IFNAR blocked-infected (b) groups at 7 dpi (n = 3). Marker M1 in the histogram represents the percentage of early apoptotic cells population. (c) Line diagram showing the percentage of early apoptotic cells in IFNAR blocked-infected (solid line), isotype-infected control (round dotted line) and non-infected control (square dotted line) groups. Results are presented as mean ± SEM at each time point (n = 3). Two-way ANOVA with Bonferroni post-test was used. *P < 0.05, **P < 0.01 and ***P < 0.001 statistically significant compared with control group. (d) Quantification of FasL gene expressions in blood, draining LN and spleen by qRT-PCR in IFNAR blocked-BTV infected (red bars) and isotype-infected control (black bars) groups compared to non-infected control group at various time points (n = 3). (e) Apoptotic cells showed green fluorescence signals in spleen at 7 dpi (n = 3). FITC, scale bar 200 µm. (f) Apoptotic cells showed green fluorescence signals in lungs at 7 dpi (n = 3). FITC, scale bar 200 µm.