Table 3.
Quantification of BTV by qRT-PCR from blood and tissue samples at different time points.
| Tissue names | Groups | Days post infection (dpi) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 2 | 3 | 5 | 7 | 9 | 12 | 15 | 18 | 21 | ||
| Blood | IFNAR blocked-infected | 2.67 ± 0.28 | 4.13 ± 0.17 | 5.08 ± 0.11 | 6.43 ± 0.19 | 5.88 ± 0.15 | 5.16 ± 0.26 | 4.45 ± 0.15 | 3.18 ± 0.18 | 2.56 ± 0.09 |
| Isotype-infected control | 0.91 ± 0.27 | 0.52 ± 0.17 | ND | ND | ND | ND | ND | ND | ND | |
| Axillary lymph node | IFNAR blocked-infected | 1.76 ± 0.16 | 3.23 ± 0.15 | 5.36 ± 0.25 | 4.87 ± 0.18 | 4.06 ± 0.28 | 2.44 ± 0.19 | 1.16 ± 0.09 | ND | ND |
| Isotype-infected control | 0.42 ± 0.35 | ND | ND | ND | ND | ND | ND | ND | ND | |
| Inguinal lymph node | IFNAR blocked-infected | 2.33 ± 0.18 | 3.89 ± 0.15 | 5.76 ± 0.22 | 5.18 ± 0.21 | 4.63 ± 0.19 | 2.56 ± 0.11 | 1.23 ± 0.14 | ND | ND |
| Isotype-infected control | 0.51 ± 0.24 | ND | ND | ND | ND | ND | ND | ND | ND | |
| Spleen | IFNAR blocked-infected | 1.26 ± 0.09 | 2.63 ± 0.11 | 3.73 ± 0.16 | 5.36 ± 0.2 | 6.22 ± 0.19 | 5.06 ± 0.11 | 4.38 ± 0.17 | 3.26 ± 0.12 | 2.58 ± 0.11 |
| Isotype-infected control | 0.75 ± 0.27 | 0.49 ± 0.17 | ND | ND | ND | ND | ND | ND | ND | |
| Lungs | IFNAR blocked-infected | ND | 0.76 ± 0.07 | 2.23 ± 0.1 | 4.78 ± 0.36 | 5.23 ± 0.28 | 4.73 ± 0.24 | 3.46 ± 0.21 | 1.63 ± 0.18 | 1.29 ± 0.13 |
| Isotype-infected control | ND | 0.41 ± 0.07 | ND | ND | ND | ND | ND | ND | ND | |
| Thymus | IFNAR blocked-infected | 1.79 ± 0.08 | 2.25 ± 0.17 | 3.76 ± 0.12 | 4.66 ± 0.14 | 4.38 ± 0.28 | 3.56 ± 0.39 | 2.85 ± 0.18 | 1.85 ± 0.25 | ND |
| Isotype-infected control | 0.66 ± 0.19 | 0.39 ± 0.28 | ND | ND | ND | ND | ND | ND | ND | |
| Heart | IFNAR blocked-infected | ND | 1.86 ± 0.06 | 3.48 ± 0.16 | 4.33 ± 0.18 | 4.28 ± 0.19 | 3.68 ± 0.13 | 2.86 ± 0.27 | 1.68 ± 0.2 | ND |
| Isotype-infected control | ND | 0.38 ± 0.26 | ND | ND | ND | ND | ND | ND | ND | |
| Brain | IFNAR blocked-infected | ND | ND | 1.86 ± 0.19 | 2.36 ± 0.16 | 3.26 ± 0.28 | 2.43 ± 0.23 | 1.09 ± 0.11 | ND | ND |
| Isotype-infected control | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Uterus | IFNAR blocked-infected | ND | ND | 1.13 ± 0.15 | 1.47 ± 0.18 | 1.25 ± 0.16 | 1.18 ± 0.15 | 0.88 ± 0.18 | ND | ND |
| Isotype-infected control | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Ovaries | IFNAR blocked-infected | ND | ND | 0.96 ± 0.15 | 1.14 ± 0.26 | 1.18 ± 0.19 | 1.02 ± 0.17 | 0.75 ± 0.09 | ND | ND |
| Isotype-infected control | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
ND: Not detected.
Virus load in blood is given as log10 copies of viral RNA ml−1 of samples and virus load in tissues is given as log10 copies of viral RNA g−1 tissue. Results are presented as mean ± SEM at each time point.