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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: J Photochem Photobiol B. 2021 May 12;220:112212. doi: 10.1016/j.jphotobiol.2021.112212

Figure 3. Red light mediated S-nitrosoprotein release from blood vessels and from BAECs.

Figure 3.

(A) Red light (670 nm, 6 J/cm2) induced dilation of blood vessel. The bath buffer was collected, centrifuged, pelleted. S-nitrosoprotein formation was detected with anti-S-nitrosocysteine antibody (green), and its co-localization with FM 5–95 membrane marker dye (red) was recognized. *p<0.05 vs. Control, #p<0.02 vs. Light. (B): BAECs were exposed to light (670 nm, 6 J/cm2) and S-nitrosoprotein formation was detected with anti-S-nitrosocysteine antibody (green). DAPI staining marks the nuclei (blue). *p<0.002 vs. Control, #p<0.005 vs. Light. Mercuric chloride was applied as specificity control for RSNO. (C): BAECs were irradiated in HBSS (670 nm, 6 J/cm2), and medium were collected. Up: Representative chemiluminescence trace. Down: Quantification with S-nitrosoglutathione standard. RSNO level increase in light treated sample was significant compared to HBSS control (&p<0.02) and ambient light control ($p<0.05). Values are mean±SE.