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. 2021 Jun 22;24(2):597. doi: 10.3892/mmr.2021.12236

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Protective effect of SNHG8 on HI/R myocardial injury. (A) Reverse transcription-quantitative PCR was used to determine the expression levels of various lncRNAs before or after HI/R injury in H9C2 myocardial cells. *P<0.05, **P<0.01. (B) Cell Counting Kit-8 assay was used to examine cell viability under different conditions. ***P<0.001 vs. Control. (C) Cell proliferation was determined by EdU analysis. **P<0.01, ***P<0.001 vs. Control. (D) LDH assay was used to detect LDH release. **P<0.01, ***P<0.001 vs. Control. *P<0.05. (E) Flow cytometric analysis of cell apoptosis in different groups. **P<0.01, ***P<0.001 vs. Control. (F) The expression levels of Bax, Bcl-2 and cleaved caspase-3 were determined by western blotting. *P<0.05 vs. Control. ^#P<0.05 vs. HI/R. FOXD3-AS1, forkhead box D3 antisense RNA1; HI/R, hypoxia-ischemia-reoxygenation; LDH, lactate dehydrogenase; siRNA, small interfering RNA; SNHG8, small nucleolar RNA host gene 8; TUG1, taurine upregulated 1.