Figure 2.

SNHG8 regulates miR-335 expression. (A) StarBase was used to predict miR-335 as a potential target of SNHG8B. (B) Relative luciferase activities of WT and Mut SNHG8 reporter plasmid in H9C2 cells after co-transfection with miR-335 mimics. **P<0.01 vs. NC mimic. (C) Schematic diagram of the relationship between SNHG8 and miR-335. (D) Reverse transcription-quantitative PCR was used to determine the expression level of miR-335 after treatment with SNHG8 siRNA. **P<0.01. miR, microRNA; Mut, mutant; NC, negative control; siRNA, small interfering RNA; SNHG8, small nucleolar RNA host gene 8; UTR, untranslated region; WT, wild-type.