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. 2021 Jun 22;24(2):597. doi: 10.3892/mmr.2021.12236

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — RASA1 mediates the protective effect of SNHG8 on HI/R myocardial injury. (A) Cell Counting Kit-8 viability assay and (B) EdU analysis of cell proliferation after transfection with RASA1 siRNA alone or after co-transfection with SNHG8 siRNA. **P<0.01, ***P<0.001 vs. Control. (C) LDH expression was determined following transfection with RASA1 siRNA alone or co-transfection with SNHG8 siRNA. **P<0.01, ***P<0.001 vs. NC. (D) The number of apoptotic cells was determined by flow cytometry. *P<0.05 vs. NC. (E) Western blot analysis of Bax, Bcl-2 and cleaved caspase-3 protein expression levels in H9C2 cells following transfection RASA1 siRNA alone or co-transfection of RASA1 siRNA and SNHG8 siRNA. *P<0.05 vs. NC. HI/R, hypoxia-ischemia-reoxygenation; LDH, lactate dehydrogenase; miR, microRNA; NC, negative control; RASA1, RAS p21 protein activator 1; siRNA, small interfering RNA; SNHG8, small nucleolar RNA host gene 8.