Fig. 2.

KEGG pathway enrichment with potential murine targets of miR-125a-5p and miR-615-3p and relative expression of mRNA and miRNA in colonic tissue of secondary abiotic IL-10^−/− mice upon C. jejuni 81–176 infection. A Gene enrichment analysis of mutual target genes of miR-125a-5p and miR-615-3p showing four significantly accumulated target genes (14.3% of associated genes) in the KEGG pathway ‘Mucin type O-glycan biosynthesis’ possessing a term P value corrected with Bonferroni step down of 0.03. B Fold changes of MUC2 and TFF3 mRNA expression were relatively calculated to naïve controls (NC) and normalised with HPRT and SDHA. C Expression levels of miRNA-615-3p and miR-125a-5p were calculated relatively to naïve controls (NC) and normalised with miR-16 and miR-145. D The relative transcript levels of ST3GAL1 and ST3GAL2 mRNA were calculated to naïve controls and normalised with HPRT and SDHA. The qPCR data were reported as the average of the relative expression in at least ten biological replicates; columns show the mean ± SD (bars). Asterisks represent a statistical significance compared to negative controls at each time point. *P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001, unpaired t-test