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. 2021 Jun 28;13:42. doi: 10.1186/s13099-021-00437-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Specific interaction of miR-615-3p with ST3GAL2 confirmed by RNAi and dual luciferase reporter assay. A, B RT-qPCR analysis for ST3GAL2 transcription in CMT 93 cells transfected with miR-615-3p mimics and nonsense miRNA as control. Fold change of ST3GAL2 and miR-615-3p was calculated relatively to nonsense control and normalised with HPRT, SDHA, B2M and miR-16, miR-145, respectively. Datasets are presented as means of three biological samples and double measurements ± SD. C Identified target sites of ST3GAL2 and miR-615-3p were analysed with RNAhybrid. D Relative luciferase activity was determined to the nonsense miRNA mimic serving as control. Luciferase signal was assayed at 24 h post-transfection. Dataset was plotted as average values of at least four biological replicates with standard deviation from three measurements. Columns show the mean ± SD (bars). Asterisks represent a statistical significance compared to negative controls at each time point. *P ≤ 0.05, ****P ≤ 0.0001, unpaired t-test