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. 2021 Jun 28;13:42. doi: 10.1186/s13099-021-00437-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Detection of protein expression of ST3GAL2 in colonic tissue of secondary abiotic IL-10^−/− mice upon C. jejuni 81–176 infection. A, B Representative micrographs comparing ST3GAL2 immunofluorescence results in the colonic tissue of naïve mice (A) and C. jejuni 81-176-infected mice (B). ST3GAL2 was immunofluorescently labelled in red and nuclei was stained with DAPI in blue. Micrographs at left side are at magnification 10 × (Scale bar 100 μm) and right side at higher magnification 40× (scale bar 25 μm) and exposure time were the same in all sections. Data are representative for three biological replications. C Right panel exemplifies detection of ST3GAL2 from three individual replicates of infected and non-infected mouse colonic tissue via Western blotting. GAPDH is shown as respective loading reference. ST3GAL2 protein bands were quantified by densitometry relative to the respective GAPDH signals. Charts represent means of at least seven biological replicates ± SD, unpaired t-test