Table 3.

Methylation levels of the OPG, RANK and RANKL genes

Gene_ID	Number of subjects		Methylation levels %		p value
	Control	Case	Control (mean ± SD)	Case (mean ± SD)
OPG1_CpG_2	49	50	0.06 ± 0.01	0.07 ± 0.01	0.046 a
OPG2_CpG_1	50	49	0.08 ± 0.09	0.13 ± 0.10	0.016 b
OPG2_CpG_5	47	47	0.05 ± 0.04	0.07 ± 0.06	0.032 a
OPG2_CpG_10.11	48	50	0.04 ± 0.02	0.05 ± 0.03	0.020 b
RANK1_CpG_6	48	49	0.16 ± 0.04	0.09 ± 0.08	0.035 a
RANK1_CpG_15	49	50	0.82 ± 0.25	0.93 ± 0.20	0.001 a
RANK1_CpG_20	49	50	0.07 ± 0.09	0.12 ± 0.15	0.005 a
RANK1_CpG_21	48	47	0.13 ± 0.17	0.23 ± 0.22	0.044 a
RANK2_CpG_10.11	46	50	0.07 ± 0.04	0.17 ± 0.19	< 0.001 a
RANK2_CpG_15	47	50	0.04 ± 0.04	0.09 ± 0.08	0.001 a
RANK2_CpG_16	46	48	0.05 ± 0.03	0.08 ± 0.02	< 0.001 a
RANK2_CpG_21.22	47	50	0.02 ± 0.01	0.03 ± 0.03	0.043 a
RANK2_CpG_23.24.25.26.27	47	50	0.04 ± 0.02	0.07 ± 0.03	< 0.001 a
RANK2_CpG_29	47	50	0.01 ± 0.02	0.03 ± 0.04	0.007 a
RANK2_CpG_30.31	45	49	0.04 ± 0.02	0.05 ± 0.01	< 0.001 a
RANK2_CpG_36	47	50	0.03 ± 0.03	0.04 ± 0.03	0.019 a
RANK3_CpG_1	46	50	0.38 ± 0.09	0.41 ± 0.07	0.023 a
RANK3_CpG_8	46	50	0.05 ± 0.03	0.09 ± 0.00	< 0.001 b
RANK3_CpG_10	47	50	0.03 ± 0.02	0.04 ± 0.01	0.007 a
RANKL1_CpG_1	43	49	0.07 ± 0.05	0.12 ± 0.11	0.009 b
RANKL1_CpG_11	45	50	0.02 ± 0.03	0.03 ± 0.02	0.014 a
RANKL1_CpG_12.13	44	49	0.10 ± 0.07	0.11 ± 0.03	0.004 a
RANKL1_CpG_19	43	49	0.07 ± 0.15	0.10 ± 0.14	0.001 a
RANKL1_CpG_21	44	50	0.06 ± 0.08	0.08 ± 0.14	0.041 a
RANKL1_CpG_24	46	50	0.11 ± 0.15	0.13 ± 0.16	0.018 a
RANKL1_CpG_25.26.27	45	50	0.30 ± 0.19	0.35 ± 0.15	< 0.001 a
RANKL1_CpG_44.45	45	50	0.03 ± 0.01	0.04 ± 0.01	< 0.001 b
RANKL2_CpG_2	48	50	0.10 ± 0.06	0.15 ± 0.09	0.016 b
RANKL2_CpG_9.10.11.12	48	50	0.05 ± 0.04	0.07 ± 0.02	< 0.001 a

p < 0.05 indicates statistical significance

^a Mann-Whitney U test

^b Independent samples t test