Fig. 5.

hPMSCs enhance Nrf2 levels but inhibit the activation of NF-κB in the mononuclear cells of GVHD mice. The mice were sacrificed to harvest the mononuclear cells of the spleen and liver after the treatment for 7 days for WB. WB was performed to determine the expression of Nrf2, HO-1, NQO1, GCLC, and GCLM in the mononuclear cells of the liver (a, b) and spleen (c, d) of GVHD mice in different groups (n = 5). WB was performed to determine the expression of phosphorylated NF-κB and I-κB in the mononuclear cells of the liver (e, f) and spleen (g, h) of GVHD mice in different groups (n = 5). The results were obtained from three independent experiments, *P < 0.05, **P < 0.01