Fig. 6.

hPMSCs induce the differentiation of CD4⁺IL-10⁺ T cells by controlling the activation of the Nrf2 and NF-κB pathways. CD4⁺IL-10⁺ T cells were induced, accompanied by changes in IL-2, IL-10, IL-27, and IFN-α2b levels in vitro, by using the naive CD4⁺ T cells treated with or without the Nrf2 inhibitor ML385 for 12 h, and then naive CD4⁺ T cells were cultured with or without hPMSCs and collected after 3 days for WB. a WB was performed to determine the expression of Nrf2, HO-1, NQO1, GCLC, and GCLM in the different CD4⁺IL-10⁺ T cell differentiation systems in vitro. b qRT-PCR analysis of the expression of Nrf2, HO-1, NQO1, GCLC, and GCLM in the different CD4⁺IL-10⁺ T cell differentiation systems in vitro. c WB was performed to determine the expression of phosphorylated NF-κB and I-κB in the different CD4⁺IL-10⁺ T cell differentiation systems in vitro. The results were obtained from three independent experiments, *P < 0.05, **P < 0.01