Figure 5.

Plantar dermis homogenate (PD) plays vital roles in promotion of sweat gland (SG) and hair follicle (HF) genesis in HF spheroids. (a) Brightfield images of HF spheroids and surrounding SG cell mass in SG scaffolds after 14-day culture of HF spheroids seeded on SG scaffolds (scale bar, 500 μm). (b) Merged brightfield and green fluorescence images of HF spheroids seeded on SG scaffolds after 14-day culture of HF spheroids seeded on SG scaffolds (green fluorescence expressed by GFP-mesenchymal stem cells (MSCs) in SG scaffolds; scale bar, 250 μm). (c) SG-specific markers detected in HF spheroids with MSCs as the seed cells in SG scaffolds after 7-day culture (scale bar, 50 μm). (d) HF-specific markers detected in HF spheroids with MSCs as the seed cells in SG scaffolds after 7-day culture (scale bar, 50 μm). Nu SG scaffolds w/o PD, PD SG scaffolds with PD, HF on MSC w/o PD HF spheroids seeded on MSC-laden three-dimensional constructs without PD, HF on MSC with PD HF spheroids seeded on MSC-laden three-dimensional constructs with PD. KRT cytokerati, Nu group means SG scaffolds without PD, PD group means SG scaffolds with PD, HF on MSC w/o PD group means HF spheroids seeded on MSC-laden three-dimensional constructs without PD, HF on MSC with PD group means HF spheroids seeded on MSC-laden three-dimensional constructs with PD