Figure 6.

Plantar dermis homogenate (PD) and 3D scaffolds without seed cells promote hair follicle (HF) and sweat gland (SG) genesis. (a) Gene expression of SG markers in HF spheroids after 7-day culture (^*p < 0.05, n = 3, unpaired Student’s t test). (b) Gene expression of HF markers in HF spheroids after 7-day culture (^*p < 0.05, n = 3, unpaired Student’s t test). (c) SG-specific markers detected in HF spheroids on alginate–gelatin gel (AG) or AG-PD scaffolds after 7-day culture (scale bar, 50 μm). (d) HF-specific markers detected in HF spheroids on AG or AG-PD scaffolds after 7-day culture (scale bar, 50 μm). 3D group means HF spheroids seeded on AG scaffolds, 3D+PD group means HF spheroids seeded on AG-PD scaffolds