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. 2021 May 11;8(3):rbab010. doi: 10.1093/rb/rbab010

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© The Author(s) 2021. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — EVs isolation and characterization. (A) Transmission electron microscopy (TEM) micrographs of isolated EVs. Representative TEM images were taken from EVs isolated from hBMSCs (MSC EVs) and 3-day osteogenic differentially hBMSCs (3d EVs). Isolated EVs were negatively stained with 1% uranyl acetate (UA) before TEM imaging. Scale bar = 100 nm (top panel); 80 nm (lower panel). (B) Nanoparticle tracking analysis for isolated EVs. EVs were diluted in PBS (1:500) for nanoparticle tracking analysis. Representative size distribution and concentration of EVs isolated from hBMSCs and 3-day osteogenic differentially hBMSCs are shown. (C) Characterization of exosome markers (CD63 and CD81) by Western blot. Lane 1: protein marker; lane 2: MSC EVs; lane 3: 3d EVs.