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. 2021 May 11;8(3):rbab010. doi: 10.1093/rb/rbab010

Table 1.

Primers used in RT-PCR

Genes Primer sequences
IL-6

Forward: 5′-ATAGTCCTTCCTACCCCAATTTCC-3′

Reverse: 5′-GATGAATTGGATGGTCTTGGTCC-3′

iNOS

Forward: 5′-CGAAACGCTTCACTTCCAA-3′

Reverse: 5′- TGAGCCTATATTGCTGTGGCT-3′

TNF-α

Forward: 5′-CTGAACTTCGGGGTGATCGG-3′

Reverse: 5′-GGCTTGTCACTCGAATTTTGAGA-3′

IL-10

Forward : 5'- GAGAA CATGGCCCAGAAATC-3′

Reverse : 5′- GAGAAATCGATGACAGCGCC-3′

CD206

Forward: 5′- AGACGAAATCCCTGCTACTG -3′

Reverse: 5′- CACCCATTCGAAGGCATTC -3′

Murine

GAPDH

Forward: 5′-TGACCACAGTCCATGCCATC-3′

Reverse: 5′-GACGGACACATTGGGGGTAG-3′

ALP

Forward: 5′-CGTGGCTAAGAATGTCATCATGTT-3′

Reverse: 5′-TGGTGGAGCTGACCCTTGA-3′

Col-I

Forward: 5′-CCCTGGAAAGAATGGAGATGAT-3′

Reverse: 5′-ACCATCCAAACCACTGAAACCT-3′

Runx2

Forward: 5′-CATGGCGGGTAACGATGAA-3′

Reverse: 5′-AGACGGTTATGGTCAAGGTGAAA-3′

OPN

Forward : 5′- GACCAAGGAAAACTCACTAC-3′

Reverse: 5′- CTGTTTAACTGGTATGGCAC-3′

BMP-2

Forward: 5′- CTGAACTCCACTAATCATGC-3′

Reverse: 5′- CTGCCCTGTTACTGCCATTATT-3′

BMPRII

Forward: 5′- GGCAGCAGTATACAGATAGGTG-3′

Reverse: 5′- CTGCCCTGTTACTGCCATTATT-3′

Human GAPDH

Forward: 5′-TCAGCAATGCCTCCTGCAC -3′

Reverse: 5′-TCTGGGTGGCAGTGATGGC -3′