Table 1.
Genes | Primer sequences |
---|---|
IL-6 |
Forward: 5′-ATAGTCCTTCCTACCCCAATTTCC-3′ Reverse: 5′-GATGAATTGGATGGTCTTGGTCC-3′ |
iNOS |
Forward: 5′-CGAAACGCTTCACTTCCAA-3′ Reverse: 5′- TGAGCCTATATTGCTGTGGCT-3′ |
TNF-α |
Forward: 5′-CTGAACTTCGGGGTGATCGG-3′ Reverse: 5′-GGCTTGTCACTCGAATTTTGAGA-3′ |
IL-10 |
Forward : 5'- GAGAA CATGGCCCAGAAATC-3′ Reverse : 5′- GAGAAATCGATGACAGCGCC-3′ |
CD206 |
Forward: 5′- AGACGAAATCCCTGCTACTG -3′ Reverse: 5′- CACCCATTCGAAGGCATTC -3′ |
Murine GAPDH |
Forward: 5′-TGACCACAGTCCATGCCATC-3′ Reverse: 5′-GACGGACACATTGGGGGTAG-3′ |
ALP |
Forward: 5′-CGTGGCTAAGAATGTCATCATGTT-3′ Reverse: 5′-TGGTGGAGCTGACCCTTGA-3′ |
Col-I |
Forward: 5′-CCCTGGAAAGAATGGAGATGAT-3′ Reverse: 5′-ACCATCCAAACCACTGAAACCT-3′ |
Runx2 |
Forward: 5′-CATGGCGGGTAACGATGAA-3′ Reverse: 5′-AGACGGTTATGGTCAAGGTGAAA-3′ |
OPN |
Forward : 5′- GACCAAGGAAAACTCACTAC-3′ Reverse: 5′- CTGTTTAACTGGTATGGCAC-3′ |
BMP-2 |
Forward: 5′- CTGAACTCCACTAATCATGC-3′ Reverse: 5′- CTGCCCTGTTACTGCCATTATT-3′ |
BMPRII |
Forward: 5′- GGCAGCAGTATACAGATAGGTG-3′ Reverse: 5′- CTGCCCTGTTACTGCCATTATT-3′ |
Human GAPDH |
Forward: 5′-TCAGCAATGCCTCCTGCAC -3′ Reverse: 5′-TCTGGGTGGCAGTGATGGC -3′ |