Figure 4.

Physicochemical characterization of the thiolated GMP groups. (A) Crosslinking density was determined as % lysine loss measured via TNBSA assay. Data is reported as means ± standard deviation for n = 3 replicates per group. *Indicates significant difference (P < 0.05). (B) Swelling ratio of the thiolated particle groups compared to GMP control. Data is reported as means ± standard deviation for n = 5 replicates per group. No statistical significance was detected (P < 0.05). (C) Degradation of the thiolated and control GMP groups in PBS at 37°C. Data is reported as means + standard deviation for n = 4 replicates per group. (D) Degradation of the thiolated and control GMP groups in collagenase-containing medium at 37°C. Data is reported as means + standard deviation. N = 4 replicates per group