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. 2021 Feb 28;8(3):rbab012. doi: 10.1093/rb/rbab012

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© The Author(s) 2021. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Quantification of MSC adherence to gelatin and thiolated GMPs and adhered MSC metabolic activity. MSC-microparticle aggregates were analysed via picogreen assay to determine (A) percent (%) adherence to the microparticles. Data is reported as means ± standard deviation for n = 3 replicates per group. No significant difference was detected between groups (P < 0.05). (B) The aggregates were cultured in rotational culture and the metabolic activity of the adhered MSCs was examined at 2 h and 24 h via WST1 assay. Data is reported as means ± standard deviation for n = 3 replicates per group. The dashed line indicates the activity of MSCs adhered to GMP controls. *Indicates significant differences within each group between the activity observed at 2 h compared to 24 h (P < 0.05)