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. 2021 May 16;8(3):rbab019. doi: 10.1093/rb/rbab019

Figure 8.

Figure 8.

In vitro culture of rat BMSCs loaded on PCL/ALMA composite scaffolds. (a) The viability of rat BMSCs in vitro cultured on PCL/ALMA composite scaffolds. Bright field images showing the contours of the composite scaffolds. Live/dead staining indicates cell activity of three individual zonal scaffolds (SL-PMA, ML-PMA and DL-PMA) and PMA-gradient composite scaffold after culture for 48 h in vitro (green, living cells labeled with Calcein-AM; red, dead cells labeled with PI). The distribution of rat BMSCs on PCL/ALMA composite scaffolds was shown in the merged images. Scale bars represent 100 μm. (b) Quantification of cell viability. Cell proliferation analysis of 3D printed PCL/ALMA composite scaffolds. (c) CCK-8 assay showing a measurable increase of rat BMSCs in four groups after 1, 3 and 7 days. (d) A similar trend is also shown in DNA content after 1, 7 and 14 days