Figure 1. . Single-phase extraction combined with high-resolution mass spectrometry provides accurate quantification of the whole blood sphingolipidome.

(A) Comparison of fortified recoveries of sphingolipids for three different extraction methods (butanol single phase, MTBE two phases and MTBE single phase). (B) The MS1 high resolution (R = 120,000) used for quantification of sphingolipids showed a high accuracy for Cer(d18:0/24:0). Both positive (C) and negative (D) mode are used for the structure elucidation of sphingolipid Cer(d18:0/24:0).

BP: Biphasic; Cer: Ceramides; M-H: Mass minus proton; NL: Neutral loss; MTBE: Methyl tert-butyl ether; SP: Single phase.