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. 2021 Jun 10;13(13):1037–1049. doi: 10.4155/bio-2021-0098

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© 2021 Wang, Xu & Mesaros

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Figure 2. — (A) The effects of alkaline hydrolysis (0.1 M KOH for 45 min) on intensities of sphingolipids, phospholipids and triglycerides. (B) The alkaline hydrolysis does improve the annotation but have no enhancement of intensity of Cer(m18:0/24:0) in DDA mode. (C) The annotated sphingolipids including Cer, ganglioside (GD3, GM1 and GM3), HexCer (Hex1Cer, Hex2Cer and Hex3Cer), SM, SPH and SPHP. (D) The annotated ceramides belong to three main subclasses: Cer, deoxyCer and phytoCer.

AA: Absolute area; Cer: Ceramides; DDA: Data-dependent acquisition; Hex: Hexosyl; ISTD: Internal standard; NL: Neutral loss; PC: Phosphatidylcholine; PE: Phosphatidylethanolamine; SM: Sphingomyelin; SPH: Sphingosine; SPHP: Sphingosine phosphate; TG: Triglycerol; RT: Retention time.