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. 2021 Jun 10;13(13):1037–1049. doi: 10.4155/bio-2021-0098

Figure 3. . Inclusion list-based parallel reaction monitoring/data-independent acquisition improves the annotation of isobaric subspecies of ceramides.

Figure 3. 

(A) The schematic graph for the strategy used for PRM/DIA analysis. (B) The isomers of Cer(d45:1) were annotated by PRM through acquiring the MS2 spectra of low abundant Cer(d19:1//25:0). (C) The MS2 spectra of Cer(d18:1/26:0) and Cer(d19:1/25:0) showed their representative fragments. (D) The co-eluted isomers Cer(d18:1/24:2) and Cer(d18:2/24:1) were separated by their representative fragments in DIA mode. (E) The final annotated sphingolipids improved by PRM/DIA, with 159 total sphingolipids. (F) The final annotated ceramides, 54.

Cer: Ceramides; DIA: Data-independent acquisition; GD: Ganglioside, disialo trihexosyl ceramide; GM: Ganglioside, monosialo trihexosyl ceramide; PRM: Parallel reaction monitoring; SM: Sphingomyelin; SPH: Sphingosine; SPHP: Sphingosine phosphate.