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. 2021 Jun 10;13(13):1037–1049. doi: 10.4155/bio-2021-0098

Figure 4. . Appropriate internal standard corrections eliminate matrix effects & achieve high dynamic linearities between peak intensity & whole blood volume.

Figure 4. 

(A) Correlation between peak intensity and blood volume, shows big matrix effects for high volumes of blood. (B) Comparison of the raw peak intensity or normalized intensity (internal standard 12:0 ceramide or 25:0 ceramide) of Cer(d18:1/20:0), and their linearities against blood volume. (C) Comparison of the raw peak intensity or normalized intensity (internal standard 12:0 Hex1Cer or C15 Hex1Cer-D7) of Hex1Cer(d18:1/20:0), and their linearities against blood volume. (D) Comparison of the raw peak intensity or normalized intensity (internal standard 12:0 SM or 18:1 d9 SM) of SM(d34:1), and the linearity against blood volume.

Cer: Ceramide; Hex: Hexosyl; SM: Sphingomyelin.