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. 2021 Jun 15;12:686060. doi: 10.3389/fimmu.2021.686060

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Copyright © 2021 Chen, Hung, Tsai, Shih, Chou, Lai, Wang and Hsueh

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Poly(I:C) stimulation altered the expression of Tlr3 and downstream genes in Tlr3^t/t spleen and cells. (A) Quantitative RT-PCR analysis of Tlr3 and the cytokines Il-6, Tnfα and Ifnβ in poly(I:C)-challenged Tlr3^t/t mouse spleen. Six hours after an intraperitoneal injection of 5 mg/kg poly(I:C), total RNA was extracted from spleens of Tlr3^t/t mice using TRIzol reagent. The expression levels of indicated cytokine genes were normalized against the internal control Hprt. (B–D) Primary cultures of BMDMs (B, D) and glia cells (C) from Tlr3^t/t mice were stimulated with 10 μg/ml poly(I:C) for 6 h. The relative RNA levels of indicated genes normalized against the internal control Hprt were analyzed by means of quantitative RT-PCR. Data are represented as mean ± SEM (error bars). Each dot indicates the result of (A) an individual animal or (B–D) an independent culture. *p > 0.05; **p > 0.01; ***p > 0.001.