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. 2021 Jun 17;220(8):e202007171. doi: 10.1083/jcb.202007171

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© 2021 Guerreiro et al.

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Figure 1. — WDR62 localizes to microtubules at spindle poles.(A and B) Immunofluorescence images of metaphase. HeLa cells recorded by confocal (z-stack) and STED (one-plane) microscopy (A) or RPE1 cells recorded by deconvolution wide-field microscopy (one plane; B); cells were stained with α-tubulin, centrin-1, and WDR62 antibodies. Insets show spindle poles. (C and D) Immunofluorescence images of metaphase RPE1 cells, treated with or without a cold treatment and stained with DAPI, WDR62 and α-tubulin antibodies, and centrin-1 (C) or pericentrin (D) antibodies. Arrows indicate depolymerized microtubule minus-ends. (E and F) Immunofluorescence images of metaphase HeLa cells treated either with DMSO or 8 nM taxol for 12 h, stained with DAPI, WDR62 and α-tubulin antibodies, and centrin-1 (E) or pericentrin (F) antibodies. Arrowheads indicate centriole- and pericentrin-free spindle poles. Scale bars = 5 µm; 1 µm (inset).