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. 2021 Jun 17;220(8):e202007171. doi: 10.1083/jcb.202007171

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© 2021 Guerreiro et al.

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Figure S3. — WDR62^−/− HeLa cells show no difference in spindle orientation or pole-to-pole distance, and spindle length of MG132-arrested RPE1 cells are constant. Related to Fig. 4. (A and B) Quantification of spindle orientation (A) and pole-to-pole distances (B) in WDR62^+/+ and WDR62^−/− metaphase HeLa H2B-GFP cells; dot plots represent median per experiments with bars displaying mean ± SEM (top) and frequency distribution (bottom): N = 5, n = 323 (WDR62^+/+) and 330 (WDR62^−/−) cells; two-tailed unpaired t test. (C) Quantification of spindle length during photoactivation (PA) experiments in indicated siRNA treatments; lines represent mean per condition, with bars displaying SEM. n = 15 cells per condition.