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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Curr Protoc. 2021 Jun;1(6):e179. doi: 10.1002/cpz1.179

Figure 2. Representative images of ventralization phenotypes at 24 hpf following chd morpholino microinjections, or exposures to vehicle (0.1% DMSO) or 4’-H (0.5–10 μM) with exposures initiated at 0.75 hpf (Basic Protocol 1).

Figure 2.

A) Normal embryo at the prim-5 stage, with yellow arrow denoting head and red arrow denoting tail. B) Class 1 ventralization with head (yellow arrow) and slightly thickened tail (red arrow). (C-D) Class 2–3 ventralization with lack of development of head (yellow arrow) and thickened blood island (red arrow) and tail. E) Class 4 ventralization with no proper head development (yellow arrow) and thick ventral tissues (red arrow) (E).