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. Author manuscript; available in PMC: 2022 Jun 10.
Published in final edited form as: Cell. 2021 May 26;184(12):3267–3280.e18. doi: 10.1016/j.cell.2021.04.035

Figure 4. RAD51 Localizes to Cas9-Induced DSBs During G2 in Zygotes.

Figure 4.

(A) Representative images of RAD51 immunocytochemistry in G1/S, S/G2, and M-phase zygotes injected with Cas9 and RAD51. Arrowheads indicate RAD51 puncta. Dotted lines highlight pronuclear nucleoli. (Scale bar=10μm) (B) Quantification of zygotes positive for RAD51 puncta (one-sided Fisher’s exact test). ***p<0.001, ****p<0.0001, n.s. p>0.05