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. 2017 Sep 15;24(1):1338–1349. doi: 10.1080/10717544.2017.1377316

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — (A) Expression of OCTN2 (SLC22A5) and ATB^0,+ (SLC6A14) proteins in MB231, MCF7 and Caco-2 cells, with β-actin as an internal control; (B) Uptake of coumarin 6 from bare nanoparticles (PLGA NPs) and L-carnitine conjugated nanoparticles (LC-PLGA NPs) in these three cell lines; (C) Effect of Na⁺ and Cl^– on the uptake of coumarin 6 from LC-PLGA NPs; (D) Effect of specific inhibitors (α-MT and glycine for ATB^0,+, L-carnitine for OCTN2) on the uptake of coumarin 6 from LC-PLGA NPs. Data are shown as mean ± SD, n = 3. *, p < .05, **, p < .01, ***, p < .001, compared to uptake in NaCl buffer (C, D).