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. 2017 Apr 24;24(1):707–719. doi: 10.1080/10717544.2017.1303855

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — (A) In vitro drug release of the free CUR, CUR-SPC NPs, PEG-CUR-SPC NPs and FA-PEG-CUR-SPC NPs in PBS. Data represents mean ± SD. (n = 3). (B) In vitro pH-dependent drug release of the FA-PEG-CUR-SPC NPs in PBS. Data represents mean ± SD. (n = 3). (C) Lysosomal colocalization of the DiD-loaded CUR-SPC NPs, DiD-loaded PEG-CUR-SPC NPs and DiD-loaded FA-PEG-CUR-SPC NPs in HeLa cells after incubation for 12 h was observed by LCSM. The hydrophobic part of NPs was stained with DiD. The nuclei were stained with DAPI. The lysosomes were stained with LysoTracker red. (The scale bars were 25 μm). (D) In vitro cellular uptake of HeLa cells co-incubated with CUR-SPC NPs, PEG-CUR-SPC NPs and FA-PEG-CUR-SPC NPs examined by confocal laser scanning microscopy after 1, 2 and 4 h incubation, respectively.