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. 2017 Apr 24;24(1):707–719. doi: 10.1080/10717544.2017.1303855

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — (A) In vitro cellular uptake of HeLa cells incubated with free CUR, CUR-SPC NPs, PEG-CUR-SPC NPs and FA-PEG-CUR-SPC NPs examined by confocal laser scanning microscopy after 4 h incubation. Blue signal, DAPI; green signal, CUR. (B) Flow cytometry histogram profiles of HeLa cells incubated with free CUR, CUR-SPC NPs, PEG-CUR-SPC NPs and FA-PEG-CUR-SPC NPs for 4 h. (C) Quantitation of mean fluorescent intensity of free CUR, CUR-SPC NPs, PEG-CUR-SPC NPs and FA-PEG-CUR-SPC NPs in HeLa cells by flow cytometry. Data were presented as mean ± SD. (n = 3, *p < 0.05). (D, E) In vitro cell viability of HeLa cells incubated with free CUR, CUR-SPC NPs, PEG-CUR-SPC NPs and FA-PEG-CUR-SPC NPs at different concentrations (0.01, 0.05, 0.1, 0.5 and 1 mg/mL) for (D) 24 and (E) 48 h. (F, G) In vitro cell viability of Caco-2 cells incubated with drug-free CUR-SPC NPs and drug-free PEG-CUR-SPC NPs at different concentrations (0.01, 0.05, 0.1, 0.5 and 1 mg/mL) for (F) 24 and (G) 48 h.