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. 2017 Dec 7;24(1):1750–1761. doi: 10.1080/10717544.2017.1402220

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Confocal laser scanning fluorescent microscopy imaging of neuromasts in 5 dpf larvae after fluorophore Texas red tagged gentamicin (GTTR) treated for 10 min. 5 dpf zebrafish larvae were pretreated by EM only (A) GGA loaded unmodified NPs (B) or GGA loaded SS31-NPs (C) for 1 h followed by the exposure of 100 μM GTTR for 10 min. Scale bar: 10 μm. (D) SS31 modified NPs significantly reduced GTTR uptake in hair cells. The mean fluorescent intensity was quantified in five neuromasts (SO1, SO2, SO3, O1, and O2) per larvae, summed to calculate one value and averaged for each group (one-way ANOVA, ^★p < .01, ^★★★p < .001).