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(A) Cell viability was detected by CCK8 assay after cells were incubated for 24 h. (B and C) Cell apoptosis was detected by flow cytometry using the Annexin V-FITC/PI double-labeling method. Date represented are mean ± SD of 3 separate experiments. **p < 0.01 versus the control group. ++p < 0.01 versus group Bup1000.
