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Cytotoxicity studies on HNE-1cells after different treatment. (A) (a) In vitro cell viability of free HK, NATNH and ATNH on HNE-1 cell after 24 h incubation; (A) (b) in vitro cell viability of BVN. *p < 0.05 and **p < 0.01, ATNH and NATNH relative to free HK. Fluorescent images of HNE-1 cells treated with medium B(a), FITC-labeled PCEC-PEI B(b), FITC-labeled PCEC-PEI-FA B(c), FITC-labeled PCEC-PEI-FA nanoparticles B(d), and FITC-labeled PCEC-PEI-FA nanoparticles + FA B(e) for 2 h.
