Table 1.

Applications and modifications of DNA tetrahedron in the field of drug delivery.

Cargo		Connective approach	Modification	Cell line in vitro	In vivo	Ref.
Small molecules	Doxorubicin	Insetting	L-DNA	SCC7 cells	Y	(Kim et al., 2016)
			Aptamer	MCF-7 cells	N	(Dai et al., 2016)
			Aptamer & Folic acid	HT-29 cells	N	(Sun et al., 2017)
			Tumor-penetrating peptide	U87MG cells	N	(Xia et al., 2016)
			L-DNA	HeLa cells	Y	(Kang et al., 2017)
	Methylene blue	Insetting	/	B16F10 cells SCC7 cells MDA-MB231 cells	Y	(Kim et al., 2016)
	Actinomycin D	Insetting	/	Escherichia coli Staphylococcus aureus	N	(Setyawati et al., 2014)
Nucleic acid	CpG	Pre-Linking	/	RAW264.7 cells	N	(Jiang Li et al., 2011)
	siRNA	Overhang	Folic acid	HeLa cells	Y	(Hyukjin Lee et al., 2012)
	ASOs	Loop	Lipofectamine 2000	Hela cells C2C12 cells MCF7 cells	N	(Keum et al., 2011)
		Insetting	PNA	Escherichia coli	N	(Readman et al., 2017)
	Aptamer	Overhang	/	HeLa cells NIH3T3 cells	N	(Charoenphol & Bermudez, 2014)
		Pre-Linking	L-DNA	HeLa cells NIH-3T3	N	(Kim et al., 2014)
		Overhang	/	MCF-7 cells A549 cells HT-29 cells	N	(Dai et al., 2016)
		Overhang	Folic acid	HT-29 cells	N	(Sun et al., 2017)

Y: yes; N: no.